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A high-throughput, plate reader-based method for the assessment of oxidative stress in suspension mammalian cells using CellROX Green July 27, 2026
The measurement of intracellular reactive oxygen species (ROS) levels provides critical insights into cell health and pathophysiology. Here, we present a semi-quantitative, plate reader-based protocol using CellROX Green that can be used for the real-time measurement of general ROS levels of viable suspension mammalian cells. With menadione being the oxidative stress inducer, a cell concentration […]
Transient receptor potential vanilloid channels in inflammatory bowel diseases: linking inflammation, angiogenesis, and oxidative stress July 27, 2026
Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is characterized by chronic relapsing intestinal inflammation driven by complex interactions among immune dysregulation, epithelial barrier disruption, vascular dysfunction, oxidative stress, and gut microbiota imbalance. Despite advances in biologic and small-molecule therapies, a substantial proportion of patients remain refractory to treatment or experience […]
Exploratory profiling of oxidative stress and hydrogen sulfide synthesis gene expression and sequence variants in gastric cancer patients July 27, 2026
CONCLUSION: Somatic mutations and altered expression of CBS, CTH, MPST, CAT, and GPX1 in GC tissues indicate coordinated remodeling of sulfur metabolism and redox-defence pathways. Nevertheless, the low ROC performance suggests that these genes should not yet be viewed as viable solo diagnostic biomarkers. Instead, they are exploratory molecular signatures and candidate markers that must […]
Multinuclear iron enzymes expand RiPP chemical diversity to enable bacterial oxidative stress defense July 27, 2026
RiPP structural complexity is significantly expanded by multinuclear non-heme iron-dependent oxidative enzymes (MNIOs). Here, we characterize pseudoprobactin 1 and 2, two MNIO-modified proteins from Pseudomonas protegens Pf-5. Using MS, NMR, and X-ray crystallography, we show that the PbnBC converts precursor cysteines into 5-thiooxazoles. While the precursors feature an N-terminal signal peptide and an intramolecular disulfide, […]
Disruption of Nrf2 signaling underlies oxidative stress and inflammatory injury induced by nitenpyram in Hyriopsis cumingii July 27, 2026
Neonicotinoid pesticides (NNIs) are emerging aquatic contaminants, yet their toxicological mechanisms in bivalves remain insufficiently understood. In this study, the effects of nitenpyram (NTP) on Hyriopsis cumingii were investigated using integrated biochemical, metabolomic, and molecular approaches. Hemocyte assays revealed dose-dependent cytotoxicity, mitochondrial dysfunction, oxidative stress, and genotoxicity, while hepatopancreatic analyses demonstrated metabolic activation, sustained...